tagE [2018-12-18 17:28:17]

UDP-glucose:polyglycerol phosphate glucosyltransferase

Locus: BSU35730

Isoelectric point: 6.03

Molecular weight: 78.12 kDa

Protein length: 673 aa

Gene length: 2019 bp

Function: biosynthesis of teichoic acid

Product: UDP-glucose:polyglycerol phosphate glucosyltransferase

Essential: no

E.C.: 2.4.1.52

Synonyms: rodD,gtaA,gtaD

Genomic Context

Loading

Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of teichoic acid
• Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of teichoic acid
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 3,678,399 → 3,680,420

Phenotypes of a mutant

• inactivation of tagE is advantageous in environments with fluctuating glucose availability PubMed

The protein

Catalyzed reaction/ biological activity

• transfer of glucose from UDP-glucose to glycerol phosphate in polymeric form PubMed

Protein family

• glycosyltransferase 1 family (according to UniProt)

Modification

• phosphorylation on Ser-2 PubMed

Structure

• 4X7R (from Staphylococcus aureus, 26% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: tagD-tagE-tagF-tagG-tagH
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PhoP: repression, PubMed, in PhoP regulon
  • WalR: activation, PubMed, in WalR regulon

  Regulation

  • repressed by phosphate (PhoP) PubMed

Biological materials

Mutant

• 1A981 ( tagE::erm), PubMed, available at BGSC
• BKE35730 (ΔtagE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTTTTACTCCCTTTCG, downstream forward: _UP4_GTCACGGAAATAAAAGAGAG
• BKK35730 (ΔtagE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTTTTACTCCCTTTCG, downstream forward: _UP4_GTCACGGAAATAAAAGAGAG

References

Reviews

Loading

Original publications

Loading